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For microbial categories, what are Aerobic and Anaerobic of microbes?
Aerobic microorganisms require oxygen to survive, such as Bacillus subtilis, fungi, etc. Anaerobic microorganisms do not need oxygen, such as lactic acid bacteria, Clostridium butyricum, etc. Facultative microorganisms can survive in both aerobic and anaerobic environments, such as Bacillus coagulans.
What are the key control factors for microbial fermentation?
It includes pH, dissolved oxygen, medium composition, stirring speed, defoaming, etc.
What is the difference between microbial activation and propagation?
Activation is mainly used in aquaculture. It refers to rejuvenating dormant Bacillus powder by adding brown sugar and aerating for 2 –4 hours. The total number of microbes does not increase, and the activation rate is 50% –60%. DME technology enables expanding hundreds to thousands of times.
What are the indicators for evaluating the quality of microbial products?
The key is the viable cell count. The higher the viable cell count, the more effective viable bacteria, and the better the product quality.
How do we examine cultured microbial liquid by microscopic? How do we distinguish different microbes?
Gram staining. It can be observed with a specific microscope (customized), no staining is needed to determine the quantity and purity.The different forms are obvious: Lactobacillus is small rod-shaped, Bacillus is medium rod-shaped, Clostridium butyricum is long rod-shaped, Enterococcus faecalis is small round, Fungal spores is large round (much larger than bacteria)
Is the inoculant a single strain or multiple strains? What strain is the inoculant?
Aquaculture is a single strain fermentation; Livestock and poultry are compound fermentation (compound lactic acid bacteria, Clostridium butyricum + Bacillus coagulans); Planting is compound bacillus; fungi is a single strain (Trichoderma harzianum, Paecilomyces lilacinus,Beauveria bassiana)
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