The long-term excessive application of chemical inputs has led to soil structure degradation, soil compaction, nutrient imbalance, and pathogen accumulation, resulting in weakened vegetable growth, reduced yields, deteriorated quality, and severe pest infestations in some plots... In Yuzhong County, Gansu Province, these issues have become critical bottlenecks constraining the development of the vegetable industry.

(Harvesting of summer vegetables on the plateau in Yuzhong County)

Yuzhong County is a major production area for plateau summer vegetables, with main crops including vegetables, corn, potatoes, wheat, lilies, and traditional Chinese medicinal herbs, accounting for over 90%. The vegetable planting area exceeds 430,000 mu (approximately 28,667 hectares).

In 2025, the Agricultural Technology Extension Center of Yuzhong County, Gansu Province participated in the "Hundred Counties, Ten Thousand Acres" Soil Health and Yield Enhancement Initiative. By adopting the "functional strain-live bacteria preparation on-site-immediate production and application" technology model, the center implemented the approach in celery and Chinese broccoli cultivation. The demonstration plots had suffered severe plant mortality and seedling rot for two consecutive years. After three applications of live bacteria, significant improvements were observed, with markedly better results compared to the control area.

The Agricultural Technology Extension Center of Yuzhong County reported that dead seedlings with yellowish-green leaves were observed in the celery plot without bacterial application, while no dead seedlings were detected in the bacterial application plot, where the leaves remained dark green and the plants exhibited robust growth.

The application of soil amendment and growth-promoting bacterial solution (Trichoderma harzianum + Bacillus subtilis) during the seedling stage of cauliflower resulted in highly significant growth differences within fourteen days, demonstrating excellent overall growth promotion performance.

In the demonstration area, the control efficacy of celery root rot, stem rot, and the diseases of Chinese cabbage (Brassica napus) including downy mildew, soft rot, and black spot exceeded 50%.

In August 2025, the National Agricultural Technology Center launched a nationwide initiative to collect critical technical needs for crop cultivation, including simplified and efficient integrated cultivation models that can effectively address the challenges of continuous cropping in protected fruit and vegetable production.

The 2025 "Hundred Counties, Ten Thousand Acres" demonstration project has validated that the green nitrogen bio-technology model—featuring functional strains, live bacteria on-demand production, and immediate application—provides an efficient and stable solution for managing continuous cropping obstacles.

Left: Bacterial machine, Right: Bacteria station

Viable microbial colonization promotes root growth within 7-10 days, resulting in well-developed root systems, robust plant vigor, and enhanced stress resistance. The soil structure is optimized, nutrient utilization efficiency improves, microbial diversity is restored, and soil-borne diseases are reduced by over 70%, effectively alleviating crop rotation issues and preventing plant death and seedling rot. This approach reduces the use of chemical fertilizers and pesticides, lowers soil pollution risks, and mitigates agricultural non-point source pollution.

• One piece of BioSmart Fermenter can produce 25 liters of live microbial liquid in 24 hours, meeting the demand for 50 mu (Approx 3.3hectares) of greenhouse cultivation.
• One piece of BioUltra Fermentation Station can generate 1,000 liters of live microbial liquid per 24 hours, meeting the demand for over 3,000 mu (approximately 200 hectares) of economic crops.